Preprint. This includes the introduction of FRT sites that may be used in the FLP/FRT system and/or Lox sites that may be used in the Cre/loxP system. Cells alter their phenotype in response to numerous environmental stimuli and can lose the expression of genes typically used to mark their identity, making it difficult to research the contribution of certain cell types to disease. official website and that any information you provide is encrypted This 3 OH group is highly unstable, and the strand on which it is present must find its complement. government site. Many Cre constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct comparison of Cre+ and Cre- cells. the loxP sites will delete the gene The Saccharomyces cerevisiae FLP-FRT recombination system is another site-directed recombination technology very conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) site being analogous to Cre and loxP, respectively. 2001 Jun 15;499(1-2):147-53. doi: 10.1016/s0014-5793(01)02541-8. 2022 Aug 26;13:964496. doi: 10.3389/fimmu.2022.964496. eCollection 2022. NNNTANNN", where "N" indicates which bases may vary from the canonical sequence, Sternberg, N. and Hamilton, D. 1981. [15][16] As of February 2019, CreLox recombination is a powerful tool and is used in transgenic animal modeling to link genotypes to phenotypes.[12][17][18]. sites contain nucleotide variations in the left or right arms, respectively named LE and RE. Now customize the name of a clipboard to store your clips. Expressed in chondrocytes, mCherry and Cre-ERT2 - Tamoxifen inducible; Targeting vector for Pax7, Venus and Cre-ERT2 with MCS for inserting promoter, Emerald and Cre-ERT2 with MCS for inserting promoter, TFP and Cre-ERT2 with MCS for inserting promoter, tdTomato and Cre-ERT2 with MCS for inserting promoter, Venus, Cre-ERT2, targeting vector with MCS for homology arms, Emerald, Cre-ERT2, targeting vector with MCS for homology arms, TFP, Cre-ERT2, targeting vector with MCS for homology arms, tdTomato, Cre-ERT2, targeting vector with MCS for homology arms, GAG-Cre fusion; for the production of VLPs loaded with CRE protein, sfGFP-iCre-ERT2 (PAPGSTM N-terminus, unskippable linker) - Tamoxifen inducible, sfGFP-iCre-ERT2 (PAPGSTM N-terminus, GSAx9 linker) - Tamoxifen inducible, iCre-ERT2 (PAPGSTMA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PV N-terminus) - Tamoxifen inducible, iCre-ERT2 (PVV N-terminus) - Tamoxifen inducible, iCre-ERT2 (PVGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PA N-terminus) - Tamoxifen inducible, CreLite - Red light-inducible Cre; N terminus Cre fused to PIF6, CreLite - Red light-inducible Cre; C terminus Cre fused to PhyBdelta, CreLite system components, PhyBdeltaCreC and PIF6CreN, in middle entry vector (Tol2 kit), CreLite; Tol2 destination vector with mTagBFP2, CreLite system components, PhyBdeltaCreC and PIF6CreN, in AAV donor/transfer vector, CreLite system components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector, Cre recombinase split with Vivid photodimers, Cre-ERT2 - Tamoxifen inducible; AAV donor vector, iCre-ERT2 (PAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAAA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAAGSA N-terminus) - Tamoxifen inducible, iCre-ERT2 (PAGSAS N-terminus) - Tamoxifen inducible, N-terminal Cre component split with Vivid photodimers, C-terminal Cre component split with Vivid photodimers, Split Cre fused to nuclear localized wild-type VVD, Retinal ganglion cell-specific expression of Cre, Cre activates shRNA expression, removal of EGFP; See also similar plasmids, Cre turns off shRNA expression, removal of EGFP; See also similar plasmids, Cre activates gene of interest by removing Stop sequences; Puro selection. While Cre-lox recombination is a very useful tool, Cre expression can be toxic in some systems, notably Drosophila. Flanking a DNA sequence with loxP sites allows for the target sequence to be manipulated by Cre using a cyclization mechanism. The site includes an asymmetric 8 bp sequence, variable except for the middle two bases, in between two sets of symmetric, 13 bp sequences. Well, it enables them to generate tissue-specific and inducible knockouts and thereby have exquisite control over the location and timing of gene expression important stuff when deleting a certain gene everywhere or during development leads to an embryonic lethal phenotype. The loxP sequence does not occur naturally in any known genome other than P1 phage, and is long enough that there is virtually no chance of it occurring randomly. Looks like youve clipped this slide to already. The use of the Cre/loxP system for generating tissue specific or inducible knockouts is a powerful tool for mouse genetics, but it is only one piece of the total picture. Cre recombinase removes the stop sequence, so the transgene is expressed only in the cells where Cre is active (see Figure 4 below). Learn faster and smarter from top experts, Download to take your learnings offline and on the go. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR, AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR, AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR, AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA, pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert, pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2, pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre, hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean, pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP, Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientists View, CREM and CMV-STOP-luc cassette, separated by insulator, Cre-EGFP fusion in a promoterless vector with several available restriction sites, tet inducible Cre-GFP fusion, metallothionein MT-I region including the polyadenylation site and several introns, Cre-ERT2 with loxp cassette; Targeting vector for Rosa26 locus, CREM fused to mutant AR ligand binding domain, Cre fused to HIV-TAT - promotes cellular uptake of recombinant Cre, Cre-Myc, Expressed in rod photorecetor cells in the retina. In general, these systems use one or more proteins and act on unique asymmetric DNA sequences. Careers. As Cre/ loxP . doi: 10.1002/jbm4.10706. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. Shortly thereafter, researchers in the laboratory of Prof. Klaus Rajewsky reported the production of pluripotent embryonic stem cells bearing a targeted loxP-flanked (floxed) DNA polymerase gene. In this fashion, opposite DNA strands are joined together. The P1 bacteriophage uses these components as part of its natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. Since homologous recombination occurs after DNA replication, two strands of DNA are available, and thus, the 3 OH group must pair with its complement, and it does so, with an intact strand on the other duplex. Tissue and sitespecific recombination in transgenic mice. he canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. 10. The RecA protein is very important for DNA repair; therefore, it is recA dependent recombination. Use of the system has greatly expanded . Cre-loxP recombination that result in insertion requires loxP sites to flox the gene of interest, with the loxP sites oriented towards each other. Models thus obtained are more likely to mimic the physiological situation. The Cre-lox recombination system prevents these situations by unlinking the rings of DNA by carrying out two recombination events (linked rings -> single fused ring -> two unlinked rings). bioRxiv. As with any other technology or research tool, it has limitations and pitfalls that need to be considered while planning experiments or interpreting results. As of February 2019, Cre-Lox recombination is a powerful tool and is used in . The enzyme (38kDa) is a member of the integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites (). Does Addgene accept orders by fax, phone or email? Clipping is a handy way to collect important slides you want to go back to later. The Cre-Lox recombination is quite a simple tool to use and manipulate, thus it has been widely used in science since the discovery of Cre more thant 30 years ago (1). I will also explain how to use Cre reporters). Removal of selectable markers from the genome by Cre-lox recombination is an elegant and efficient way to circumvent this problem and is therefore widely used in plants, mouse cell lines, yeast, etc.[1]. Efficient Cre/loxP site-specific recombination . 2. Cre-RMCE is functional in a two-step mechanism and with good efficiency in Ae. The Cre recombinase is fused to a mutant form of the oestrogen receptor, which binds the synthetic oestrogen 4-hydroxytamoxifen instead of its natural ligand 17-estradiol. Govt. outcome. You can read the details below. These will be your experimental mice. Diversity and Selection of Shell of the Hermit Crab of Mandvi, Kachchh Coast, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Explanation: The Cre recombinase protein of bacteriophage P1 mediates site-specific recombination at a 34 bp sequence, loxP. In this edition, were going to take a look at a very interesting tool that can be used for creating (excuse the pun)specific, targeted DNA modificationsin transgenic animals, embryonic stem cells, and/or tissue-specific cell types:Cre-lox recombination. 2. Unable to load your collection due to an error, Unable to load your delegates due to an error. 11. If there exists a floxed donor sequence, the donor sequence can be swapped with the original sequence. Use inducible cre gene embedded into the lox construct. [38], A classical strategy for generating gene deletion variants is based on double cross-integration of non-replicating vectors into the genome. CRY2-CreN and mCherry coexpression; Light inducible; Use with plasmid 26889. There is a problem with the plasmid I received. It is a member of a large family of tyrosine recombinases that includes several other familiar recombinases, such as Flp and Int. Hepatic Nampt Deficiency Aggravates Dyslipidemia and Fatty Liver in High Fat Diet Fed Mice. [13][14] To date, NIH Blueprint for Neuroscience Research Cre projects have created several hundreds of Cre driver mouse lines which are currently used by the worldwide neuroscience community. Read Addgene's Blog on Cre alternatives from Gerald Rubin's lab. Comparative mitochondrial zygomycetes: bacterial-like Rnase P RNAs, mobile el No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. mouse), and another with the loxP sites (the floxed mouse) are created, and crossed to. Depending on your experimental parameters, you can use these as controls (see Figure 3A below). Cre/ lox is usually used to make knockout alleles, but it can also be used to activate gene expression. See, Cre activates gene of interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration; See similar plasmid. In the lysogenic cycle the phage genome replicates with the rest of the bacterial genome and is transmitted to daughter cells at each subsequent cell division. (RBE); the two RBE are 13bp This system can be implemented without inserting any extra supporting proteins or sequences. Plasmids. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. [19], Cre recombinase can be synthesized by the corresponding gene under the direction of cell-specific promoters, including promoters under the control of doxycycline. Studies have found that Cre recombinase is the best The temperature is 37 oC and Flp recombinase is 30. First, the nucleotide sequence identity in the spacer region of lox site. It appears that you have an ad-blocker running. How can I be notified when a plasmid from a specific lab or paper is available? The virus, called a prophage in such a state, accomplishes this via integration and excision. To account for this, alternate mutant versions of the loxP site have been created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which bases may vary from the canonical sequence. instance, if two loxP sites are placed on different chromosomes, a translocation can take found in the yeast and based on the yeast two-micron plasmid. systemhas two components: Cre recombinase derived from bacteriophage P1 two34- bp lox Crerecognizes; site-specificrecom- bination Cre-mediatedcatalysis re-ciprocal recombination between twolox bothtissue culture cells mice[18,19 . Cre-Lox recombination is a site-specific recombinase technology widely used to carry out deletions, insertions, translocations and inversions in the DNA of cells. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 2ZZSB1121 through backcross conversion and/or transformation, and to the . The exact sequence is given below; 'N' indicates bases which may vary, and lowercase letters indicate bases that have been mutated from the wild-type. Among these are loxN (GtATACcT), lox2272 (GgATACtT), and lox511 (GtATACAT). Inducible Cre activation is achieved using CreER (estrogen receptor) variant, which is only activated after delivery of tamoxifen. Expressed in limb connective tissue, TFP and Cre-ERT2 - Tamoxifen inducible. OMP targeting vector with Cre FNF (neo-selectable marker flanked by FRT sites), M71 targeting vector with IRES CreFNF (neo-selectable marker flanked by FRT sites), Cre, Puro resistance and miRNA expression. Again, only one generation of breeding is required. This event can have three general outcomes based on the location and orientation of the loxP sites: Inversion: If the loxP sites are on the same DNA strand and are in opposite orientations, recombination results in an inversion and the region of DNAbetween the loxP sites is reversed. In part 1 of this mini-series, I introduced the concept and applications of Cre-loxP. Cre-lox, conditional genetics; cre; cre-loxP; gene editing; loxP; recombination; site directed mutagenesis. The Cre-lox system serves several functions in the phage: it circularizes the phage DNA into a plasmid, separates interlinked plasmid rings so they are passed to both daughter bacteria equally and may help maintain copy numbers through an alternative means of replication. With genomic tumor testing, a negative result is not always definitive. Accessibility Nov 2, 2015. [7] Combining these advances in collaboration, the laboratories of Drs. Disclaimer. Matsumura T, Watanabe T, Totsugawa T, et al. The schematic below shows the three types of rearrangements: inversion, deletion and translocation. 2017 by John Wiley & Sons, Inc. Keywords: Thus, the orientation, and location of the loxP sites are important determinants of KAUSHAL KUMAR SAHU The strands are then rejoined with DNA ligase in a quick and efficient process. Fill out our Suggest a Plasmid form or e-mail [emailprotected] to help us improve this resource! Addgene is a nonprofit plasmid repository. During site-specific DNA recombination, which brings about genetic rearrangement in processes such as viral integration and excision and chromosomal segregation, these recombinase enzymes recognize specific DNA sequences and catalyse the reciprocal exchange of DNA strands between these sites. Cre/lox switchingexpression system Currently, mostwidely used site-specific DNA recombination system system[18, 19]. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. 2023 Feb 10;12(4):568. doi: 10.3390/cells12040568. Lox sites are directional and the two sites joined by the tetramer are parallel in orientation. 2007;(178):29-48. doi: 10.1007/978-3-540-35109-2_2. We've updated our privacy policy. Cell Culture Media, Introduction to the Cre-Lox Recombination System, AAALAC-accredited and OLAW assured animal centers, Specific-pathogen-free (SPF) animal health status exceeds requirements of most facilities, Top-tier customer support: free consultations and quotes. IHDR y ] + sRGB pHYs od FIDATx^.{s" ""S Tetrad analysis, positive and negative interference, mapping through somatic Chromosome walking jumping transposon tagging map based cloning, Conversion of fibroblasts to retinal cells by transcription (final). To verify that Cre recombinase expression does not contribute to a phenotype of interest, I suggest using thecrestrain itself (without anyloxP-flanked alleles) as a control (Figure 3B). By Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. We take a look at the history of genomic sequencing, where it's going in the future, and the power it has to potentially treat diseases like cancer. (Already know this? Federal government websites often end in .gov or .mil. The site is secure. I highly recommend that you check out his blog. The P1 phage is a temperate phage that causes either a lysogenic or lytic cycle when it infects a bacterium. The Cre enzyme leads to the recombination between lox sites removing the marker gene along with one lox site. These variant lox sites undergo recombination with other sites of the same type, but are not cross-compatible. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Approximately 25% of the progeny from this mating will be homozygous for theloxP-flanked allele and hemizygous/heterozygous for thecretransgene. The circular molecules contain site-specific recombination sequences and allow transformation of host cells with DNA comprising only selected sequences of interest. Furthermore, the Cre ERT gene is knocked in to the Axin2 locus in Axin2-Cre ERT mice, Both 13bp repeat sequences on a single loxP site are recognized and bound by aCre protein, forming a dimer. The Cre-lox recombination system has been particularly useful to help neuroscientists to study the brain in which complex cell types and neural circuits come together to generate cognition and behaviors. The Cre protein (encoded by the locus originally named as "Causes recombination", with "Cyclization recombinase" being found in some references)[21][22] consists of 4 subunits and two domains: The larger carboxyl (C-terminal) domain, and smaller amino (N-terminal) domain. The Cre/lox system can be used to turn off a transgene. Cre Recombinase - 1 ul (1 unit*) H20 - Xul (up to 50ul) Incubate at 37C for 30 minutes and then 70C for 10 minutes. OurTechnical Information Services (TIS)is receiving more and more requests for help in designing breeding schemes for producing Cre/loxmice. Front Immunol. lox. The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system. Bethesda, MD 20894, Web Policies 8600 Rockville Pike <The exam for Jae Lee's sessions> Please provide a ' brief and concise ' answer to each question. The Cre-lox system is used to introduce deletions, insertions, translocations, and inversions at a specific genomic locus. A novel maize variety designated 2ZZSB1121 and seed, plants and plant parts thereof are provided. RuvC is just one of these Resolvases that have been isolated in bacteria and yeast. Sternberg, N. and Hamilton, D. 1981. See, Heat-inducible reporter with Cre dependent H2B-RFP expression, Switches from DsRed to eGFP expression upon the presence of Cre, Zsgreen is expressed in cells without Cre, mCherry is expressed with Cre, Double fluorescent, double selectable cre/loxP reporter, Reporter construct for lineage tracing and mosaic analysis in zebrafish. Fields, Pathways recombination is reversible, strategies for irreversible integration rely on mutated . place. The Cre/lox system can also be used to produce strains in which a transgene is either inducible or expressed only in certain tissues. 18 Digvijay Autonomous P. G. College Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene expression. The Cre recombinase can recognize cryptic sites in the host genome and induce unauthorized recombination, damaging host DNA. Dre-Rox system. Enhanced fungal resistance in transgenic cotton expressing an endochitinase g University Research Meeting Poster Apr 2014. Another report using two lox variants pair but through regulating the length of DNA in one pair results in stochastic gene activation with regulated level of sparseness. In this example, expression of Cre recombinase removes the transgene (Figure 6). [8] They observed 50% of the DNA polymerase beta gene was deleted in T cells based on DNA blotting. Maize hybrid X13C737 is an invention by Andrew Jon Ross, Lisbon IA UNITED STATES. Gene editing in monogenic autism spectrum disorder: animal models and gene therapies. The Cre or cre-recombinase protein (called so because it causes recombination) catalyzes site-specific recombination events between two DNA recognition sites. We've encountered a problem, please try again. Colorful Retro Vintage Illustration Animated Medical Technology Education Sci Quantized space time and Einstein's special theory of relativity.pdf, Doodle Astronomy Lesson XL by Slidesgo.pptx. Two plasmids can be joined using the variant lox sites 71 and 66. The Cre/lox system is a well-established research tool, especially in the field of mouse transgenics. Increasing the length of DNA leads to decreased efficiency of Cre/lox recombination possibly through regulating the dynamics of the reaction. Approximately 50% of the offspring will be heterozygous for theloxPallele and hemizygous/heterozygous for thecretransgene. The gene of interest lies In this method, the marker gene gets removed due to the induc- Structure of the Cre-loxA complex evaluation of unintended effects, ignoring the fact that conven- besides the lox sites. The Cre- lox recombination system is derived from the bacteriophage P1. inverted repeats. It addresses the limitations of cre-loxP technology and related considerations for experimental design, and it discusses alternative strategies for site-specific genetic recombination and integration. Non-parallel recombination is especially problematic in a fate mapping scenario where one recombination event is designed to manipulate the gene under study and the other recombination event is necessary for activating a reporter gene (usually encoding a fluorescent protein) for cell lineage tracing. This review provides an overview of the cre-loxP system and its various permutations. The Cre-lox system is a technology that can be used to induce site-specific recombination events. Learn more, Download our file to copy and paste plasmid data, Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments, Basic analysis for a user-entered sequence; includes restriction sites and map, Digital collection of empty plasmid backbones from publications and commercially available sources. Chief among these is Cre recombinase, discovered in the P1 bacteriophage and named as such because it c auses re combination (Cre) between specific DNA sequences referred to as loxP (locus of crossover (x) in P1) sites, each of which are 34 base pairs in length 1. Using different lox site variants allows Cre to catalyze more than one specific recombination event in a single system. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. The SlideShare family just got bigger. 2. PubMed, Orban, P.C., Chui, D., and Marth, J.D. The two loxP sites then align in a parallel orientation, allowing the four Cre proteins to form a tetramer. CreER(T2) resides within the cytoplasm and can only translocate to the nucleus following tamoxifen administration, allowing tight temporal control of recombination. Phages like the lambda phage use their site specific recombinases to integrate their DNA into the host genome during lysogeny. [3][4][5] Subsequently, researchers in the laboratory of Dr. Jamey Marth demonstrated that Cre-Lox recombination could be used to delete loxP-flanked chromosomal DNA sequences at high efficiency in specific developing T-cells of transgenic animals, with the authors proposing that this approach could be used to define endogenous gene function in specific cell types, indelibly mark progenitors in cell fate determination studies, induce specific chromosomal rearrangements for biological and disease modeling, and determine the roles of early genetic lesions in disease (and phenotype) maintenance.[6]. For a more thorough introduction, check out Addgenes Cre-lox blog post. This can also take place in the absence of the Cre lox system[39] with the help of other bacterial and viral proteins. & Engineering, Model The result of recombination depends on the orientation of the loxP sites. Rafik Karaman. PubMed PMID:1495975. These developments have led to a widespread use of conditional mutagenesis in biomedical research, spanning many disciplines in which it becomes a powerful platform for determining gene function in specific cell types and at specific developmental times. Since both the transposon and the Cre- lox recombineering systems were applied to diverse hosts, CRAGE enabled insertion of large biosynthetic gene clusters into 25 diverse gammaproteobacterial species. This provides the advantage that the Cre recombinase is active for a short time. Cre Recombinase Cyclization recombinase (Cre) is one of the tyrosine site-specific recombinases, which is known to catalyse the site specific recombination event between two DNA recognition sites (LoxP sites). Here is Part 2!). You may not be able to create an account or request plasmids through this website until you upgrade your browser. Commonly used gene replacement strategies introduce selectable markers into the genome to facilitate selection of genetic mutations that may cause growth retardation. Cre-lox , , knockout , . In more recent years, however, this view has been challenged, and most of the current models for Int, Xer, and Flp recombination involve only limited branch migration (13 base pairs of the Holliday intermediate), coupled to an isomerisation event that is responsible for switching the strand cleavage specificity. It appears that you have an ad-blocker running. (<U>G</U>ene-<U>A</U>uto-<U>E</U>xcision <U>B</U>inary <U>S</U>ystemGAEBS)(transcription system) . [26], Holliday junctions and homologous recombination, Implementation of multiple loxP site pairs. And, it can be used to turn transgene expression on or off, track individual cells or cell lineages (as in theBrainboworConfettimice), generateinversionsortranslocations, and report gene expression. This is also the catalytic site of the enzyme. A novel maize variety designated X03R618CYFR and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. 2001 Aug 17;311(3):453-9. doi: 10.1006/jmbi.2001.4888. The Cre/lox system is one of the most powerful and versatile tools developed for mouse genetics. Embodiments include the seeds of soybean 4826008, the plants of soybean 4826008, to plant parts of soybean 4826008, and methods for producing a soybean plant produced by crossing soybean 4826008 with itself or with another soybean variety.

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